Dog Cell Lines
Dog Cell Lines kdorfman Fri, 09/01/2023 - 15:06| Line # | breed | defrost | frozen? | # swabs | notes |
|---|---|---|---|---|---|
| 104 | Newfoundland (M) | 8/28 | Very few adherent cells as of 9/1 | ||
| 104 | Newfoundland (M) | 8/31 | 10 4 from spun down culture medium |
huge numbers of floating cells (made swabs from liquid) | |
| 105 | golden | 8/28 | 6 from spun down culture medium | very few cells overall, adherents are round | |
| 106 | Maltese | 8/28 | 8/30 | 15 3 10/23/23 |
huge numbers of cells. put into 2 25 cm2 flasks>. tested regular DMEM in 12.5 cm2 flask. 1 25 cm2 flask to freeze |
| 109 | terrier mix? | 8/28 | about 2 adherent cells per field of view at 10x | ||
| 110 | pointer | 8/28 | single digits adherent cells | ||
| IPC 366 1 | ? | 8/28 | 9/1 | 9 | grew like gangbusters! 3 swabs per 12.5 cm2 flask. froze one thawed and grew up for swabs. Buckets of DNA!! (multinucleate). Froze 2 more vials. |
| 112 | Yorkshire terrier 11 YO F | 9/8/23 | 6 15 from trypsinized T75 flask |
Grow great! thawed and grew, made swabs, then they petered out. Not able to amplify enough to freeze another vial. |
|
| 113 | Beagle (Thelma) 6YO F | 9/12/23 | 10 from trypsinized T75 flask | great growing cell line, but started to fail after split. Maybe seeded too thinly? Split them all to a smaller flask 9/27 to see if they will grow up to large enough numbers to freeze |
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