Week 04

Week 04 kdorfman Mon, 07/24/2023 - 20:50

Yeast Labs week of 9/25/23

(See also seedling observations)

Pipetting exercises:

DNA extraction from yeast

  • Reagents
    • 200 mM LiOAc, 1% SDS (4x 100 uL/pair)
    • 95% EtOH (4 X 300 uL/pair)
    • 70% EtOH ( 4 X 400 uL/pair)
    • TE ( 4 x 80 uL/pair)
  • Equipment
    • microfuge tubes
    • hot block at 70C
    • centrifuges for 1.5 mL tubes, 15,000 x g
    • paper towels
    • Nanodrop

Sequences

  • send out for sequencing?
  • 4 strains per table
  • really? or pull sequences out of her back pocket?

Check MV replica plate from last week

  • check diploids
  • draw picture
  • label
  • transfer
    • some??? to YED (to check the next day, next day toothpick to YEKAc plate 1/pair (=48)
    • pick one to YED (class "pre-spore")

YED ?2 per pair? = 48

YEKAc??