Week 04

Yeast Labs week of 9/25/23

(See also seedling observations)

Pipetting exercises:

• Colorimetric pipetting exercise
• Weighing water
  • 1 balance per pair
  • weigh boat
  • beaker of dH2O
  • extra batteries

DNA extraction from yeast

• Reagents
  • 200 mM LiOAc, 1% SDS (4x 100 uL/pair)
  • 95% EtOH (4 X 300 uL/pair)
  • 70% EtOH ( 4 X 400 uL/pair)
  • TE ( 4 x 80 uL/pair)
• Equipment
  • microfuge tubes
  • hot block at 70C
  • centrifuges for 1.5 mL tubes, 15,000 x g
  • paper towels
  • Nanodrop

Sequences

• send out for sequencing?
• 4 strains per table
• really? or pull sequences out of her back pocket?

Check MV replica plate from last week

• check diploids
• draw picture
• label
• transfer
  • some??? to YED (to check the next day, next day toothpick to YEKAc plate 1/pair (=48)
  • pick one to YED (class "pre-spore")

YED ?2 per pair? = 48

YEKAc??