2023 Project 1

2023 Project 1 kdorfman Wed, 03/29/2023 - 14:33

W 3/29/23

Treatment	Stock concentration	Dilute in
Latrunculin	1 mM	serum-free medium (e.g., Fluorobrite)
Taxol	10 mM	medium
Nocodazole	33 mM	DMSO to 1 mM, medium to 3.3 uM
STLC	1 mM	medium
GSK-923295
MG132

Fixatives:

Media:

Stains:

2023 Project 1 day 2

2023 Project 1 day 2 kdorfman Wed, 03/29/2023 - 21:31

For M 4/3/23

Grp	dish or coverslip	strain	reagents
1	3 dishes	GFP tubulin	GSK,
2	3 coverslips	GFP tubulin	GSK, fix & DAPI
3	4 dishes	GFP tubulin	Taxol, nuc blue
4	6 coverslips	GFP tubulin	Latrunculin, serum-free medium, phalloidin, fix & DAPI
5	4 dishes	GFP	STLC, DMSO, nuc blue
6	6 coverslips	parental	MG 132, fix, anti alpha tubulin, goat anti-rat-FITC, DAPI
7	3 coverslips	GFP	Taxol, fix & DAPI
8	6 coverslips	GFP	MG132, methanol, hec 1 & DAPI

2023 Project 1 day 3 kdorfman Mon, 04/03/2023 - 20:57

W April 5

Group	Cells	dish or coverslip	reagents
1	GFP	3 coverslips
2	nothing	all set
3	GFP	6 dishes	Taxol, nuc blue
4	GFP tubulin	6 coverslips	Latrunculin, serum-free medium, phalloidin, fix & DAPI
5	GFP	2 dishes
6	no cells	all set	anti alpha tubulin, goat anti-rat-FITC, DAPI
7	GFP	3 coverslips
8	GFP	6 coverslips

2023 Project 1 day 4 kdorfman Wed, 04/05/2023 - 20:26

for Monday 4/10/2023