1/2 MS low Iron

1/2 Murashige & Skoog, 1µM iron medium

MS 10x micronutrients is 100 µM FeSO4, so MS complete is 10 µM, 1/2 MS is 5µM

FeSO4 stock is 10 mM, which is 10,000x 1 µM

To make	plates low-iron 1/2 MS
start with	mL ddH2O (~ 60% final volume)
add:	mL 10X macronutrients
add:	mL boric acid 1000X
add:	mL cobalt chloride 10,000X
add:	mL cupric sulfate 10,000X
add:	mL ferrous sulfate 10 mM
add:	mL KI 10,000X
add:	mL manganese sulfate 1000X
add:	mL moybdic acid 10,000X
add:	mL zinc sulfate 1000X
add:	g MES, pH to 5.7 with KOH
bring to	mL final volume
add:	g bacto- or phyto-agar
autoclave for	minutes