CMBL 2022
CMBL 2022 kdorfman Mon, 01/31/2022 - 12:33reagents
- 1M DTT (25 ml; filter sterilized and stored at -20)
- 50% PEG 3350
- 1M Lithium Acetate pH 7.5
- Qiagen miniprep kits
- Yeast beta-galactosidase assay kit (Thermo Fisher #75768--3 kits)
plates
- SC-leu high adenine plates (350 plates; we will use 50 on 2/4, 50 on 2/11, and 200 on 2/21)
- SC-leu low adenine plates (75 plates; we will use 50 on 2/11 and 25 on 2/25)
- old plates of any kind (25-30...these are just for practicing spotting cultures and maintaining sterility)