Student Protocol

  • crush leaf tissue in 600 µL 2X CTAB extraction buffer in microfuge tube using blue pestle

  • Incubate 60C 30 min. Invert 2x

  • Add 600 µL chloroform:isoamyl alcohol (24:1)

  • Centrifuge 5 min

  • Harvest supernate. (If it's cloudy, repeat chloroform:isoamyl step)

  • Add equal volume ice cold isopropyl

  • invert gently until 2 phases mix.

  • Store at -20C (at least 30 min, better overnight)