PCR Calcs

From Takara Ex Taq recipe

For 1µL PCR rxn. For PCR rxns
aliquotµL 10X Ex Taq buffer µL total
aliquotµL dNTP 2.5mM µL total
aliquotµL primer 1 12.5 µM µL total
aliquotµL primer 2 12.5 µM µL total
aliquotµL template DNA (~250 ng/µL) µL total
addµL water to final volume µL total
aliquotµL Ex Taq 1U/µL µL total

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pcrcalcs.xls27.5 KB