Mueller-Hinton agar

90922 Mueller Hinton Broth 2 from Sigma

22 grams per liter, consisting of:

  • Casein acid hydrolysate 17.5
  • Beef extract 3.0
  • Starch 1.5

Final pH 7.3 +/- 0.2 at 25°C

Add 17 g agar per L

Make 3.5 L in a 4L flask: (yields up to 140 plates)

77 g broth mix

stir till dissolved. Leave stir bar in

59.5 g agar

autoclave 80 minutes

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sigma-mueller-hinton.pdf2.18 MB