Materials

Equipment

  • microfuge tubes with blue pestles
  • 60C drybath
  • ice
  • microfuge

Chemicals:

Aliquot 600 µL each:

  • CTAB buffer
  • isopropanol (ice cold)

Aliquot 750 µL chloroform:isoamyl alcohol.

More than one/student for repeat extraction

CTAB extraction buffer

Recipe

ingredient concentration amount per L
Tris pH 8 1M 100 mL
NaCl 8.1 % 81.8 g
EDTA 0.5 M 40 mL
CTAB 2% 20 g
Beta ME 0.2% 2 mL

Buy it from Fisher: Teknova C2190 CTAB Extraction Solution:

ingredient concentration
Tris pH 8 100 mM
NaCl 1.4 M
EDTA pH 8 20 mM
CTAB 2%

Add Beta ME just before aliquoting: 2 µL/mL

to make: mL CTAB Extraction Buffer
at this concentration: %
add: µL Beta mercaptoethanol