Enzymes & kits

  • Takara Ex-Taq
    giant economy size: RR001B 4x 250U $590 at Clontech
    $843 at Fisher


Fisher equivalent has this buffer: Tris-HCl 100mM, pH 9, KCl 500 mM, MgCl2 15 mM. Takara buffer is 20 mM MgCl2

  • try this? cheap Taq at http://www.bulldog-bio.com/bioreadyrtaq.html

  • Invitrogen 18080044 superscript III 200U/µL 10KU @ $259

  • Invitrogen 10777019 RNAse out 5KU @ $143

  • Invitrogen RNAse A Check supply.

  • Qiagen 79254 DNAse
    includes RNase-free water and RDD for diluting the DNAse

  • Qiagin RNEasy 74904

    • if needed RW1 1053394
    • if needed RLT 79216
  • Quantifast 204054 400 rxns for q pcr

  • Zymo Clean & Concentrator kit

  • Oligo-dT Fisher FERSO131 Oligo dT 100 µM 60 µL @ ~$50. Dilute to 50µM!! (for emergencies: 50 µM oligo(dT) Fisher stockroom: C1101 0.5µg/µL x 40µL = 20µg)

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en-rnase-free-dnase-set-handbook.pdf52.85 KB