Concanavalin A

  • Sigma C5275 5 mg
  • Plant mitogen
  • Lectin (carbohydrate-binding protein) extracted from the jack-bean, Canavalia ensiformis
  • Con A binds specifically to α-Mannose, α-Galactose structures found in sugars, glycoproteins and glycolipids

Concanavalin-coated coverslips

  • Make stock 5 mg/mL solution (add 1 mL sterile PBS to bottle – gently rotate to mix)
  • Keep sterile - work in sterile hood
  • aliquot 100 µL into microfuge tubes (cannot take repeated freeze-thaw cycles)
  • Coat coverslip bottom dishes with
    • 0.75 mL 0.1 mg/mL: 0.015 mL (5 mg/mL) + 0.735 mL PBS
    • 0.75 mL 0.5 mg/mL: 0.075 mL (5 mg/mL) + 0.675 mL PBS
  • Incubate the coverslip dishes for 1 hour 37˚C
  • Remove the solution
  • rinse in PBS
  • Air dry the coverslip.
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concanavalin_a.pdf80 KB