3.3 Salk phenotypes

M 3/5/12

Per Pair:

  • Control seeds (aliquot ~25 into 12 microfuge tubes, dry)

  • Salk-line seeds (remainder from Lab 3.2, dry, ~25 per tube)

  • 2 mL 70% EtOH, 0.05% TritonX-100 (stock is 10%)

    • Make 50 mL, aliquot into microfuge tubes

    • 37 mL 95% EtOH

    • 0.25 mL 10% Triton-X
    • water to 50 mL
  • If seeds are to be plated in agar:

    • 0.1% agar, sterile
    • ~ 2 mL per pair
    • 0.1 g agar/100 mL, autoclave, aliquot.
    • Or aliquot into glass tubes, then autoclave
  • sterile water

  • If seeds are to be dried and plated from filter paper:

    • Filter paper (? 4 in a plastic Petri dish?)
    • Sterile forceps in tubes for handling filter paper
    • 10 mL 95% EtOH (aliquot 25 15mL tubes)
  • 2 complete medium plates per pair

  • 2 Low-Fe medium plates (1 µM Fe) per pair

  • micropore tape

Total plates (actually need 20 of each type)

  • 24 complete MS medium

  • 24 low Fe MS medium

AttachmentSize
salt-germination-10.xlsx147.71 KB

Comments

Watch plants throughout the semester

Change the lab manual so it says they have to follow any homozygous or heterozygous Salk plants throughout their entire life - till the end of the semester.