Genetics & Evolution lab

Here I have posted the overall proposed DRAFT of the lab schedule for the Genetics and Evolution Lab, as well as write-ups for the individual activities. Any and all critiques/suggestions are welcome. THANKS!

AttachmentSize
Genetics & Evolution Schedule Draft45.71 KB
Who's the Father?298.23 KB
Genotype Phenotype Analysis of Red Onion656.65 KB
Yeast Complementation laboratory88.46 KB
Ames Test136.5 KB
Virtual FlyLab112.77 KB
Per 3 Gene Polymorphisms97.51 KB
Drug Resistant TB SNP Analysis664.86 KB
DNA Fingerprinting978.13 KB
Woolly Mammoth1.31 MB
Next-Generation Sequencing Activity297.88 KB

CTAB DNA extraction

Materials

Equipment

  • microfuge tubes with blue pestles
  • 60C drybath
  • ice
  • microfuge

Chemicals:

Aliquot 600 µL each:

  • CTAB buffer
  • isopropanol (ice cold)

Aliquot 750 µL chloroform:isoamyl alcohol.

More than one/student for repeat extraction

CTAB extraction buffer

Recipe

ingredient concentration amount per L
Tris pH 8 1M 100 mL
NaCl 8.1 % 81.8 g
EDTA 0.5 M 40 mL
CTAB 2% 20 g
Beta ME 0.2% 2 mL

Buy it from Fisher: Teknova C2190 CTAB Extraction Solution:

ingredient concentration
Tris pH 8 100 mM
NaCl 1.4 M
EDTA pH 8 20 mM
CTAB 2%

Add Beta ME just before aliquoting: 2 µL/mL

to make: mL CTAB Extraction Buffer
at this concentration: %
add: µL Beta mercaptoethanol

Student Protocol

  • crush leaf tissue in 600 µL 2X CTAB extraction buffer in microfuge tube using blue pestle

  • Incubate 60C 30 min. Invert 2x

  • Add 600 µL chloroform:isoamyl alcohol (24:1)

  • Centrifuge 5 min

  • Harvest supernate. (If it's cloudy, repeat chloroform:isoamyl step)

  • Add equal volume ice cold isopropyl

  • invert gently until 2 phases mix.

  • Store at -20C (at least 30 min, better overnight)

Yeast

2/20/16

AttachmentSize
img_2923.jpg87.9 KB
img_2924.jpg88.19 KB
img_2925.jpg85.64 KB
img_2926.jpg124.74 KB
img_2927.jpg91.51 KB
img_2928.jpg89.57 KB
img_2929.jpg80.04 KB
img_2930.jpg80.97 KB
img_2931.jpg88.66 KB
img_2932.jpg88.27 KB
img_2933.jpg72.38 KB
img_2934.jpg86.05 KB