organelle slides

Alpha actinin

Stains adhesions and stress fibers

  • Monoclonal, BM 75.2
  • Fix in Formaldehyde, no glut or meOH
  • 1:200
  • IgM, use mouse FITC secondary that recognizes IgMs

Gamma tubulin

Stains Centrosomes

  • Monoclonal, GTU-88, Sigma,
  • Fixation: methanol; para/glut
  • Dilution 1:100

Golgi 58K

  • Sigma G2404, mouse monoclonal
  • Formaldehyde (no glut)
  • 1:100

Hec1

Stains kinetochores

  • Novus biological, recombinant human HEc1, monoclonal
  • Fix in methanol
  • 1:200

LAP2

Stains nuclear envelope

  • Methanol
  • 1:100
  • BD transduction
  • Mouse IgG

Vinculin

Focal adhesion plaques

  • Monoclonal, sigma V4505
  • Formaldehyde
  • 1:100