slides 2015

LL's for student slides

Fix 90 in formaldehyde; 10 in methanol

Plate 102 in 17 6-well plates.

Lab # coverslips fix stain secondary
1.1 10 form or paraglut
1.2 10 "
1.3 10 "
2.1 10 " anti-tubulin, phalloidin goat anti-rat
2.2 10 " anti-tubulin goat anti-rat
3.1 10 " anti-tubulin, phalloidin FRESH goat anti-rat
3.2 3 form alpha actinin anti-mouse IgM
3.2 3 form anti Golgi anti-mouse IgG
3.2 3 form vinculin
3.2 3 MeOH anti gamma-tubulin
3.2 3 " anti-Hec1
3.2 3 " LAP2
3.3 20 form or paraglut un-mounted for students to stain

organelle slides

Alpha actinin

Stains adhesions and stress fibers

  • Monoclonal, BM 75.2
  • Fix in Formaldehyde, no glut or meOH
  • 1:200
  • IgM, use mouse FITC secondary that recognizes IgMs

Gamma tubulin

Stains Centrosomes

  • Monoclonal, GTU-88, Sigma,
  • Fixation: methanol; para/glut
  • Dilution 1:100

Golgi 58K

  • Sigma G2404, mouse monoclonal
  • Formaldehyde (no glut)
  • 1:100

Hec1

Stains kinetochores

  • Novus biological, recombinant human HEc1, monoclonal
  • Fix in methanol
  • 1:200

LAP2

Stains nuclear envelope

  • Methanol
  • 1:100
  • BD transduction
  • Mouse IgG

Vinculin

Focal adhesion plaques

  • Monoclonal, sigma V4505
  • Formaldehyde
  • 1:100