Ionomycin for 6.2

Ionomycin stock

Fisher or Invitrogen (Life Technologies) I24222

1 mM in DMSO or EtOH. 1 mg makes 1.34 mL stock.

3 µM Ionomycin in HBS
Dilute stock 1:333 in HBS: 3 µL/1 mL

24 µL in 8 mL, aliquot 800 µL

They only need 750µL, because they do one experiment in HBS, then two in Ca-free HBS.

3 µM Ionomycin in Ca-free HBS
Dilute stock 1:333 in Ca-free HBS: 3 µL/1 mL
45 µL in 15 mL

Takes a long time to thaw. Vortex.

Aliquot 1.55 mL so they can do two 750 µL experiments

Vasopressin for 6.2

1 mg/mL (= 0.9225 mM) Vasopressin stock in water


1 µM dilution to make student solutions

1 µL 1 mg/mL in 922.5 µL water (NO CALCIUM, so stock can be used for Ca-free solutions )


10 nM (= 1.084 µg/mL) Vasopressin in HBS for students

Use Vasopressin aliquots from Lab 6.1


Calcium Free Vasopressin 10 nM

10 nM (= 1.084 µg/mL) for students

1 part 1µM dilution to 99 parts Ca-free HBS

Lab 7.2: 0.75 mL per coverslip x 2 coverslips x 8 groups = 12 mL

Make 15 mL: 150 µL + 15 mL Ca-free HBS

Make 8 aliquots of 1.6 mL