3.3 2013

M 9/30

Labeling Cellular Structures

Cells

  • paraglut fixed LL's for students to stain for tubulin (make extra!)

  • paraglut fixed LL's for students to stain for phalloidin

  • paraglut fixed 3t3's for students to stain for phalloidin

  • live LL's to fix in paraglut (2014: treat first with mitotracker, then fix in formaldehyde no detergent)

Solutions

Materials

  • forceps

  • 50 mL beakers (switch to holders and 100 mL beakers?)

  • petri dishes for humid chambers

  • filter paper for humid chambers

  • mounting medium

  • slides

  • nail polish

  • kimwipes

  • parafilm