Resistance 2013

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antibiotic_resistance_week_1_prep.docx73.18 KB
antibiotic_resistance_week_2_prep.docx66.6 KB
antibiotic_resistance_week_3_prep.docx78.76 KB
welcome_to_the_resistome_lab_manual.pdf1.07 MB

Antibiotics 2013

(weight indicates how much should be on one disk.)

Calculate concentration from volume needed per disk (25 uL)

Make 35 mL of each (because the filter syringe only holds that much)

See recipes in stock solutions.

Make 35 mL each, make 48 0.7 mL aliquots

Antibiotic ug/disk mg/mL category solubility Fisher #
Ampicillin 10 0.4 beta-lactam 50 mg/mL in 1 M NH4OH
tetracycline 30 1.2 tetracycline 10 mg/mL in water
cipro 5 0.2 quinolone 30 mg/mL in water
Kanamycin 30 1.2 Aminoglycoside 50 mg/mL in water

cipro solubility http://www.inspiralis.com/go/antibiotics.php 30 mg/mL in water

ampicillin solubility 50MG/ML IN 1M AMMONIUM HYDROXIDE http://www.sigmaaldrich.com/catalog/DataSheetPage.do?brandKey=SIGMA&symb... ampicillin sodium salt is soluble 50 mg/mL in water http://www.bioxys.com/i_gentaur/ampicillin_sodium.htm

Kanamycin 50 mg/mL in water

Tetracycline 10 mg/mL

The HCl forms are more water soluble

Plate Tally 2013

type poured remaining used
LB 222 145 77
McC 1006 280 726
M-H 1150

Resistance 1 - 2013

Jan 29 & 31, 2013

Resistance 2 2013

  • 20 MacConkey plates per student

  • sterile swabs

  • gloves

  • spray ethanol

  • markers

Resistance 3 2013

  • 26 Mueller Hinton plates

  • sterile swabs

  • 26 1-mL aliquots physiological saline

    0.9% NaCl (=0.9 g/100 mL = 9 g/L)

  • sterile sticks

  • parafilm

  • markers

  • gloves

  • micropipettors & tips

  • vortex

  • antibiotic solutions in freezer

  • sterile disks

  • sterile forceps

  • rulers

  • microtube racks