Soution A

Solution A

100 mM Tris-HCl pH 7.5

100mM EDTA

100 mM NaCl

0.5% SDS

Stock solution 10 25 50 100 mL final volume
1 M Tris 1 2.5 5 10 mL
0.5 M EDTA 2 5 10 20 mL
5 M NaCl 0.02 0.05 0.1 0.2 mL
20% SDS 0.25 0.625 1.25 2.5 mL

Make 10 mL

pH to 7.5