API Natural Trace

African Cichlid naturally derived trace minerals

Use 66 µL/L

Same for crayfish tank.

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Calculations

for cichlids (as per bottle):

5 mL/gal x 0.264 gal/L = 0.132 mL/L = 132 µL/L

Divide in half for FW mussels and crayfish

66 µL/L

Artificial Pond Water for Mussels

from http://www.biolbull.org/cgi/reprint/181/1/175.pdf

Use for partial water changes in mussel "aquaria"

Salt	1X (mM)	10X (mM)	100X (mM)
CaCl2	0.4	4	40
KCl	0.05	0.5	5
NaCl	0.5	5	50
NaHCO3	0.2	2	20

pH 7

100 X from stock solutions

80 mL aliquots to dilute to 8 L in carboy.

3 boxes changed daily for 3 weeks

Stock solution	ci (M)	cf (M)	80	800	1280	1600	final vol (mL)
NaCl	5	0.05	0.8	8	12.8	16	mL
KCl	1	0.005	0.4	4	6.4	8	mL
CaCl2	1	0.04	3.2	32	51.2	64	mL
NaHCO3 1	1	0.02	1.6	16	25.6	32	mL
OR dry NaHCO3	MW=84	0.02	0.134	1.344	2.15	2.688	g

pH 7

Filter sterilize (NaHCO3 may not be stable during autoclaving)