Solutions

Solutions to have at the beginning of the semester

Amount conc solution
100 mL 10% Tween in water
50 mL 10% Tween in PBS
100 mL 10% Triton-X in water
100 mL 10% Triton-X in PBS
5 L 10X PBS
1 L 1x PBS in 10 mL sterile aliquots
4 L 1x PBS-Tw-Az
25 mL 10% Sodium azide
250 mL 10X HBS
100 mL 10 X Ca-free HBS
500 mL 1x Non-CO2 medium with serum
1000 mL 1x Non-CO2 serum free medium
2 L 1x F10-Hams
250 mL 1x serum free F-10 Hams
1 L 1x DMEM
20 mL DAPI Fluoromount Fisher OB010020 2 bottles to share.