5.1 - 5.3 expression

5.1 - 5.3 expression kdorfman Tue, 12/20/2011 - 19:45

5.1 Microarray resources M 4/2/12

MAKE SURE GENEVESTIGATOR ACCOUNT IS ACTIVE!!

5.2 Statistical analysis of microarray data W 4/4/12

Tell students what chemicals are available

  • ABA (2014)
  • ammonium nitrate
  • 1aminocyclopropanecarboxylic acid (converts to ethylene) (dry and frozen stock)
  • kinetin
  • giberellic acid
  • salicylic acid
  • IAA (dry and frozen stock) Indole-acetic acid
  • chitin (dry) (to mimic fungus attack)
  • lots of inorganic salts

http://www.phytotechlab.com/searchresult.aspx?categoryid=10

http://www.plantmedia.com/categories/3_43_288_691/Tissue-Culture-Hormon…

paper on germination in salt: http://www.researchgate.net/publication/255710805_The_effect_of_salt_st…

5.3 RT-PCR Planning M 4/9/12

  • 2-week old seedlings on plates - 2 plates per group (plates to refrigerator F 3/23, to growth chamber M 3/26)
  • 1-week old seedlings on plates 1 - 2 plates per group (for transfer experiments) (plates to refrigerator 3/30, to growth chamber 4/2)

  • 10 microfuge tubes with sterile, imbibed seeds in small batches for germination experiments (enough in each tube for one plate) (4/6)

  • 1 L MS in 100 mL aliquots for students to pour plates and add drugs or hormones, etc.)

2014 projects

2014 projects kdorfman Thu, 04/10/2014 - 21:27

RPR

4 MS plates plain
4 MS plates + final conc 140 mM NaCl


CTH

Salicylic acid in EtOH

Dilute into tween20 in water

Spray on plants


TVZ

Colloidal solution of chitin

mortar and pestle


CCM

Spray plants with Salicylic acid. see CTH.


MOH

damaged roots vs intact roots

plates


BHK

seeds

germination wet vs dry


WRD

light vs dark


NAL


CVM

KCl