5.6 RT-PCR analysis

Make enough for 2 gels/pair 1.5% agarose

gels mL TAE g agarose µL EtBr
11 550 8.25 5.5
20 1000 15 10
22 1100 16.5 11
24 1200 18 12

Use EtBr TAE

Use LMW mass ruler