Stock solutions

Freezer

antibiotics

antibodies

Inventory

<

div id="main">

<

div id="main-inner" class="clear-block with-navbar">

<

div id="content">

<

div id="content-inner">

              <div id="content-header">
                  <div class="breadcrumb"><a href="/biology/">Home</a> › <a href="/biology/faculty">Faculty</a> › Current Faculty</div>                                      <h1 class="title">Current Faculty</h1>
                                                      </div> <!-- /#content-header -->

    <div id="content-area">
      <div class="view view-faculty-directory view-id-faculty_directory view-display-id-page_1 view-dom-id-1">
    <div class="view-header">
  <p>Below is an alphabetical listing of Current Biology faculty. Please note you can search by first or last name. All of Biology faculty are affiliated with at least one of the four <a href="/biology/graduate/interdisciplinary-graduate-programs">interdisciplinary graduate programs</a> and you can limit your search to only those faculty affiliated with a particular program.  In addition, you can search for only those faculty who can chair graduate student Masters or PhD committees.  And you can combine these criteria to further limit your search.</p>
</div>

  <div class="view-filters">
  <form action="/biology/faculty/faculty-listing"  accept-charset="UTF-8" method="get" id="views-exposed-form-faculty-directory-page-1">

  <div class="view-content">
  <table width="95%" class="views-table sticky-enabled cols-3">
<thead>
<tr>
          <th class="views-field views-field-title">
      Name        </th>
          <th class="views-field views-field-field-title-value">
      Title        </th>
          <th class="views-field views-field-teaser">
      Research Interests        </th>
      </tr>

Lynn Adler

Associate Professor

Ecology and evolution of insect-plant interactions

R. Craig Albertson

Assistant Professor

Evolutionary Developmental Biology

ABA

(+)-cis,trans-abscisic acid

Plant Media 30631017-1 250 mg $68

soluble in EtOH, MeOH, DMSO 20 - 50 mg/mL

MW = 264.3

Store as powder in freezer.

Make 1 mL of 100mM solution:

0.1mol/1000mL x 164.3g/mol = 0.0264 g

Working concentration ~0.15 mM

In 30 mL MS agar, =

vi = 30mL x 0.15 mM/100 mM = 0.045 mL

ATP

10 mM ATP Stock
(from Maniotis)

60 mg ATP in 8 mL H2O
pH to 7.0 with 0.1 M NaOH
Bring volume to 10 mL with H2O

Aliquot and freeze.

Use at ~10 µM

Adenine

Fisher AC147440250 25 gr.

1 mg/mL solution for yeast MV-Ade medium

heat and stir for a couple of hours

Antibiotics

Ampicillin

50 mg/mL stock

(Final concentration in medium = 50 µg/mL)

0.5 g in 10 mL water

filter sterilize

1 mL aliquots (enough for 1 L medium)

0.4 mg/mL for QSB

Make 35 mL:

0.28 mL 50 mg/mL stock

35 mL sterile water

(filter sterilize if any doubt about sterility)

48 0.7 mL aliquots

to make: mL amp solution
at this concentration: mg/mL
add: g dry ampicillin

Carbenicillin

(More stable than ampicillin)

Fisher 50841234b 25 mL Teknova C2130

100 mg/mL stock

effective concentration 50 - 100 µg/mL in medium

Freeze aliquots.

Chloramphenicol

MP 19032

store dry at RT

Cipro

Ciprofloxacin 10 mg/mL stock

(final concentration in medium = 10 µg/mL)

100 mg in 10 mL dilute acid (add HCl drop by drop, mixing in between till it dissolves)

filter sterilize

1 mL aliquots

freeze

0.2 mg/mL for QSB

0.7 mL 10 mg/mL stock

35 mL water

filter sterilize

48 0.7 mL aliquots

To make mL 0.2 mg/mL cipro
you'll need: g cipro

freeze

Doxycycline

Tetracycline category

solubility 50 mg/mL in water

disk has 30 ug

Erythromycin

solubility

50 mg/mL in ethanol

10 mg/mL stock

(10 µg/mL in medium)

0.25 g in 25 mL EtOH

1 mL aliquots

freeze

0.6 mg/mL for QSB

2.1 mL 10 mg/mL stock

33 mL EtOH1

48 0.7 mL aliquots

freeze


  1. Try 20 µL in 330 µL water to see if it dissolves. If so, then make aqueous solution, filter sterilize, and freeze. 

Kanamycin

50 mg/mL stock (50 µg/mL in medium)

1.25 g in 25 mL H2O

filter sterilize

1 mL aliquots

freeze

1.2 mg/mL for QSB

0.84 mL 50 mg/mL stock

34.16 mL water

filter sterilize (if any doubt about sterility)

48 0.7 mL aliquots

To make mL 1.2 mg/mL kanamycin
you'll need: g kanamycin

freeze

Kan calcs

if Kanamycin stock is: mg/mL
to make: mL medium
with a final Kan concentration of: mg/mL
Add: mL kanamycin stock

Tetracycline

15 mg/mL stock

0.275 g tetracycline

25 mL EtOH

1 mL aliquots

freeze

1.2 mg/mL for QSB

2.8 mL 15 mg/mL stock

32.2 mL EtOH1

48 0.7 mL aliquots

freeze

To make mL 1.2 mg/mL tetracylcine
you'll need: g tetracycline


  1. Try 28 µL in 322 µL water to see if it dissolves. If so, then make aqueous solution, filter sterilize, and freeze. 

Triclosan

Irgasan

Sigma 72779-5g-f

MW 289.54

Boric acid

Boric acid (H3BO3) 0.01 M (=10 mM)

1000x for M&S micronutrients

MW = 61.83

0.031 g/50 mL

MW of Boric acid:
Concentration: M
to make: mL
add: g boric acid

Calcium chloride

CaCl stocks in two concentrations:

MW (dihydrate) = 147

MW:
Concentration: M
to make: mL
add: g CaCl2

Calcium nitrate

Ca(NO3)2.4H2O

FW = 236.1

0.4M stock:

94.4g/L = 0.189g/50mL

MW of calcium nitrate:
Concentration: M
to make: mL
add: g calcium nitrate

Cholesterol

5 mg/mL for C. elegans medium

Fisher AAA1147018 Alfa Aesar 50 g ~$35

in 95% ethanol

Stir for 3 hours

or vortex several minutes

Do not autoclave!

Cobalt Chloride

Cobalt Chloride (CoCl2) 105 µM (=~ 0.1 mM)

10,000X for M&S micronutrients

MW (hexahydrate) = 237.93

0.00125 g/50 mL

MW:
Concentration: M
to make: mL
add: g dry stuff

DTT

Dithiothreitol

154.25 MW

1M = 1.5425g in 10 mL H20

MW:
Concentration: M
to make: mL
add: g DTT

EDTA

EDTA (disodium) 0.5 M pH 8.0

MW = 372.4

18.62 g/100 mL

9.31 g/50 mL

initial pH = ~6. Use NaOH pellets to bring to pH = 8. ~2 g NaOH/100 mL

MW:
Concentration: M
to make: mL
add: g EDTA

EGTA

EGTA 0.5M

100 ml solution

19g EGTA (MW 380g/mol) ddH2O to 90ml adjust pH 7.5/8.0 with solid NaOH (>4g) adjust volume to 100ml

MW:
Concentration: M
to make: mL
start with: mL
add: g EDTA, adjust pH to 7.5 or 8 with NaOH pellets, then bring to final volume

Note: EGTA will not go into solution without NaOH. Once the pH has been raised sufficiently it dissolves quickly. For pH 7.5 the exact amount required is slightly above 4g. Add 3.5-4g immediately, then proceed carefully not to overshoot the desired pH.

from http://www.researchgate.net/post/How_can_I_dissolve_EGTA

Ferric citrate

Ferric citrate (C6H5FeO7) 89.4 mM

MW=244.95

1.095 g/50 mL

0.328 g/15 mL

Keep refrigerated

The 89.4 value is because Tobias Baskin's lab makes a 89.4 µM Fe-citrate medium, and it's easy to mix up a batch from the 1000x stock solution.

Maximum solubility is 1 g/100 mL hot H2O

MW of ferric citrate:
Concentration: M
to make: mL
add: g ferric citrate

Could in the future make a 10 mM batch with 0.1225 g in 50 mL, and for this lab: https://wahoo.nsm.umass.edu/content/reagents-61 , you would have easier calculations.

Ferrous sulfate

Ferrous sulfate (FeSO4) 10 mM

MW (heptahydrate) = 278.01

MS 10x = 28mg/L = 0.1 mM - 100 µM

MS 1x = 0.01 mM = 10 µM

make 1,000x for M&S medium = 0.01 M = 10 mM

MW of FeSO4:
Concentration: M
to make: mL
add: g FeSO4

Keep refrigerated

Turns yellow. Probably oxidation. Yellow comes off on a micropore filter.

Lithium Chloride

LiCl 6M

MW = 42.39

MW of LiCl:
Concentration: M
to make: mL
add: g LiCl

Gets hot!!

MES

MW = 195.2

150 mM stock solution for LPGM

MW of MES:
Concentration: M
to make: mL
add: g MES

Magnesium chloride

Magnesium chloride (MgCl2) 1M

MW (hexahydrate) = 203.31

10.166 g/50 mL

20.33 g/100 mL

MW of MgCl2:
Concentration: M
to make: mL
add: g MgCl2

Magnesium sulfate

Magnesium sulfate (MgSO4) 1M

MW (heptahydrate) = 246.48

MW of MgSO4:
Concentration: M
to make: mL
add: g MgSO4

Manganese chloride

MnCl2 4H2O

M.W.197.9

5 mM

0.0495g/50mL

MW of MnCl2:
Concentration: M
to make: mL
add: g MnCl2

Manganese sulfate

MnSO4

MW (monohydrate) = 169

store dry in dessicator

MS 10X micronutrients: 16.9 mg/L = 0.1 mM

so 1X = 0.01mM = 10 µM

1000X MS = 10 mM

MW of MnSo4:
Concentration: M
to make: mL
add: g MnSO4

Mannitol

MW = 182.172

Concentration: %
to make: mL
add: g mannitol

Potassium acetate

Potassium acetate (C2H3O2K)

Fisher BP364-500 $46.30

MW = 98.14

49 g in 100 mL H2O

MW of KOAc:
Concentration: M
to make: mL
add: g KOAc

Potassium chloride

Potassium chloride (KCl)

MW = 74.56

MW of KCl:
Concentration: M
to make: mL
add: g KCl

Potassium ferricyanide

Potassium ferricyanide (K3Fe(CN)6) 50 mM (= 0.05 M)

red salt

MW = 329.26

0.8232 g/50 mL

MW of Potassium ferricyanide:
Concentration: M
to make: mL
add: g Potassium ferricyanide

Store frozen

Potassium ferrocyanide

Potassium ferrocyanide (K4Fe(CN)6) 50 mM (= 0.05M)

yellow salt

MW (trihydride) = 422.41

MW of Potassium ferrocyanide:
Concentration: M
to make: mL
add: g Potassium ferrocyanide

Store frozen

Potassium hydroxide

KOH

MW = 56.11

MW of KOH:
Concentration: M
to make: mL
add: g KOH

Use for adjusting PIPES pH

Potassium nitrate

Potassium nitrate (KNO3)

MW of KNO3:
Concentration: M
to make: mL
add: g KNO3

Store in refrigerator

Potassium phosphate dibasic

K2HPO4

MW = 174.18

MW of K2HPO4:
Concentration: M
to make: mL
add: g K2HPO4

Potassium phosphate monobasic

Potassium phosphate monobasic (KH2PO4)

MW of KH2PO4:
Concentration: M
to make: mL
add: g KH2PO4


for C. elegans medium:

pH to 6.0 with solid KOH

(~1.7 g/100 mL)

Sterilize (can be autoclaved)

SDS

SDS 20%

Sodium dodecanesulfate (=Sodium lauryl sulfate, NOT laureth)

CH3(CH2)11OSO3Na

Concentration: %
to make: mL
add: g SDS

DO NOT AUTOCLAVE - it (like all detergents) can boil over

Sodium Ferric EDTA

NaFe(III)EDTA

FW = 367.05

50 mM

MW of NaFe(III)EDTA:
Concentration: M
to make: mL
add: g NaFe(III)EDTA

Sodium acetate

Sodium acetate (NaOAc) 3M

CH3COONa

Make new for 2016-2017

MW (trihydrate) = 136.08

MW of NaOAc:
Concentration: M
to make: mL
add: g NaOAc

pH 5.2

Sodium azide

For PBS-Tw-Azide

NaN3

MW = 65

10% w/vol

Concentration: %
to make: mL
add: g NaN3

Sodium bicarbonate

Sodium bicarbonate (CHNaO3) 0.5 M (=500 mM)

MW = 84.01

MW of Sodium bicarbonate:
Concentration: M
to make: mL
add: g sodium bicarbonate

Write the date on it. Probably only stable for 2 weeks.

Sodium chloride

Sodium Chloride (NaCl) 5 M

MW = 58.44

Concentration: M
to make: mL
add: g NaCl

Sodium phosphate dibasic

MW of Na2HPO4:
Concentration: M
to make: mL
add: g dry stuff

Use hot water

Sodium phosphate monobasic

Sodium phosphate monobasic (NaH2PO4)

MW (monohydrate) = 137.99

MW of NaH2PO4:
Concentration: M
to make: mL
add: g dry stuff

Sodium sulfate

Na2SO4.10H2O

MW = 322.2

MW of Na2SO4:
Concentration: M
to make: mL
add: g Na2SO4

Spermidine

Tris

Tris 1M

Tris base MW = 121.14

MW of Tris:
Concentration: M
to make: mL
add: g Tris

pH to 7.5 or 8.0, depending on the application

Triton X

Triton X 10%

MW = 646.86

1 g = ~1 mL

Concentration: %
to make: mL
start with: mL Triton X

Tween 10%

Tween 10%

for PBS-Tween-Azide

1 g = ~1 mL [density = 1.095 g/mL at 25 °C (from Sigma website)]

Concentration: %
to make: mL
start with: mL Tween

Do not autoclave